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Exchange 2003 to 2010 error

Hello I have been working through a upgrade of exchange 2003. I am at a point where when I try to send from exchange 2003 to 2010 I get the following error

You do not have permission to send to this recipient.  For assistance, contact your system administrator.<My2003server.COM #5.7.1 smtp;550 5.7.1 Unable to relay for test@email.com

I have set the relay settings on my 2003 smtp server to allow all as a test with no luck any ideas?


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